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PLASMID PROFILING OF MULTIDRUG RESISTANT ESCHERICHIA COLI STRAINS ISOLATED FROM URINARY TRACT INFECTION PATIENTS

Sabin Khadgi¹, Uddhav Timilsina^1*, Basudha Shrestha²
1Department of Biotechnology, College for Professional Studies, LBEF Campus, Kathmandu, Nepal
²Department of Pathology, Kathmandu Model Hospital, Kathmandu, Nepal
Corresponding author: timilsinau@gmail.com

 

ABSTRACT
Introduction- Urinary tract infection is a common community-acquired bacterial disease. Escherichia coli is reported to be the major cause of urinary tract infection.  Aim & Objective- The study was conducted with the aim of determining the antibiotic resistance pattern and plasmid profile of multidrug resistant Escherichia coli isolated from Urinary Tract Infection patients. Materials and Method- Antibiotic susceptibility tests were performed against E. coli following the protocol for the Kirby-Bauer disc diffusion method. Plasmid DNA was isolated following the protocol of Kado and Liu. Results- Multidrug resistant isolates exhibited high resistance to drugs like Amoxicillin, Cefixime, Ciprofloxacin, Cotrimethoxazole, Norfloxacin and Ofloxacin. The plasmid profiling showed that all, except one, isolate contained at least one plasmid. A band of approximately 23 kb was seen in most of the isolates.

Key words - urinary tract infection, Escherichia coli, antibiotic resistance, plasmid profile, antibiotic susceptibility

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