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STUDY ON THERMOPHILES REVEALS THE PRESENCE OF ACTINOBACILLUS LIGNIERESII IN CATTLE COMPOST
Gagandeep Singh Saggu1* and Divya Shrivastava2
1 Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Rajasthan, India
2 School of Life Sciences, Jaipur National University, Jaipur, Rajasthan, India
*Corresponding Author email: firstname.lastname@example.org
Introduction- Moderate environment is important to sustain life any environment condition that can be perceived as beyond the normal acceptable range is extreme condition and if we are talking in the terms of temperature than its called thermophillic environment. Aim & objective- The study was designed to study the diversity of thermophiles present in compost and characterization of the properties which are responsible for their adequate nature to sustain high temperature. Material and method- Microorganisms were isolated from cattle compost and initially screened by biochemical tests to characterize till genus level. Confirmation of Actinobacillus spp. was done by fermentation reactions of nine carbohydrates and amplification of 16S rRNA region. Results- Isolates from compost showed wide range of growth temperatures which suggest their important activity during the whole composting process. This study initially designed to study the diversity of thermophiles in compost which turned specifically to A. lignieresii.
Key Words: compost, thermophiles, Actinobacillus, biochemical, molecular analysis.